Fasn Knockout cell line (AML12)

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Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Skeleton, Articulus, Soft Tissue, Derma System

Ocular, Otolaryngologic and Oral System

Cell Monoclonal Culture Medium

Monoclone Genotype Validation Kit

Fasn Knockout cell line (AML12) Order

Catalog#

YKO-H1070

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

Fasn Knockout cell line (AML12)

Morphology

Epithelial-like, adherent

Passage ratio

1：3~1：6

Species

Human

Gene Name

Fasn

Gene ID

14104

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM/F-12+10%FBS+1%ITS Liquid Media Supplement+40ng/ml Dexamethasone

Target region

Knockout region: Exon 2 (based on transcript Fasn-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:AML12				STR Info (Cell bank) Cell Line:AML12
Loci	Allele1	Allele2	Allele3	Allele4	Allele1	Allele2	Allele3
4-2	238.09 【20.3】				238.08 【20.3】
5-5	335.69 【14】	339.78 【15】			335.95 【14】	339.97 【15】
6-4	290.87 【16】				290.82 【16】
6-7	334.62 【12】				334.65 【12】
9-2	221.52 【15】				221.6 【15】
12-1	238.45 【19】				238.52 【19】
15-3	197.06 【21.3】				197.23 【21.3】
18-3	172.81 【21】				172.91 【21】
X-1	404.71 【26】				405.34 【26】
D4S2408

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the Fasn gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the AML12 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, Fasn gene knockout Fasn Knockout cell line (AML12) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

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